Monitoring mitochondrial [Ca2+] dynamics with rhod-2, ratiometric pericam and aequorin
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چکیده
منابع مشابه
Monitoring mitochondrial [Ca(2+)] dynamics with rhod-2, ratiometric pericam and aequorin.
The dynamics of mitochondrial [Ca(2+)] ([Ca(2+)](M)) plays a key role in a variety of cellular processes. The most important methods available to monitor [Ca(2+)](M) are fluorescent dyes such as rhod-2 and specifically targeted proteins such as aequorin and pericam. However, significant discrepancies, both quantitative and qualitative, exist in the literature between the results obtained with d...
متن کاملrhod-2, ratiometric pericam and aequorin
The dynamics of mitochondrial [Ca 2+ ] ([Ca 2+ ] M) plays a key role in a variety of cellular processes. The most important methods available to monitor [Ca 2+ ] M are fluorescent dyes such as rhod-2 and specifically targeted proteins such as aequorin or pericam. However, significant discrepancies, both quantitative and qualitative, exist in the literature between the results obtained with diff...
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Dynamic changes in intracellular calcium concentration in response to various stimuli regulates many cellular processes such as proliferation, differentiation, and apoptosis(1). During apoptosis, calcium accumulation in mitochondria promotes the release of pro-apoptotic factors from the mitochondria into the cytosol(2). It is therefore of interest to directly measure mitochondrial calcium in li...
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Mitochondria have a very large capacity to accumulate Ca(2+) during cell stimulation driven by the mitochondrial membrane potential. Under these conditions, [Ca(2+)](M) (mitochondrial [Ca(2+)]) may well reach millimolar levels in a few seconds. Measuring the dynamics of [Ca(2+)](M) during prolonged stimulation has been previously precluded by the high Ca(2+) affinity of the probes available. We...
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Previously, we reported that both the bradykinin (Bk)-induced increase in mitochondrial ATP concentration ([ATP]M) and the rate of cytosolic Ca2+ removal are significantly decreased in skin fibroblasts from a patient with an isolated complex I deficiency. Here we demonstrate that the mitochondrial Ca2+ indicator rhod-2 can be used to selectively buffer the Bk-induced increase in mitochondrial C...
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ژورنال
عنوان ژورنال: Cell Calcium
سال: 2010
ISSN: 0143-4160
DOI: 10.1016/j.ceca.2010.07.001